全ゲノム DNAメチル化解析 "Genome-wide DNA methylation analysis"
MFMT protocol is now opened in the journal, ProsOne..
"Genome-Wide DNA Methylation Analysis Reveals Phytoestrogen Modification of Promoter Methylation Patterns during Embryonic Stem Cell Differentiation."
The MFMT method is a comprehensive DNA methylation analysis that assesses the methylation level of the linker-ligated MspI fragments. HpaII analyzes a methylationlevel of the cytosines on the protruding ends, and McrBC assesses that of the cytosines in the fragment-body region. This complementary assay system gives researches more reliable data for the methylation assessment of MspI fragment of genome DNA..
The regular type of the MFMT analysis uses the two dyes for tiling DNA arrays..